Path: utzoo!utgpu!watserv1!watmath!uunet!snorkelwacker!apple!bionet!dulruu51.bitnet!HARTLAUB
From: HARTLAUB@dulruu51.bitnet
Newsgroups: bionet.molbio.methds-reagnts
Subject: PCR * Enzymes cut ss-DNA?
Message-ID: <9002051654.AA05140@net.bio.net>
Date: 3 Feb 90 18:25:00 GMT
Sender: daemon@genbank.BIO.NET
Lines: 9


Can anybody help me ?

Does anybody know whether larger fragments than the usual 20- to 30-mers
of oligonucleotides can be used as primers for the PCR ?
  (My aim is to connect a certain marker-gene (ca. 1500 bp) instead of a
   restriction-site directly to the PCR-fragments to avoid cloning.)

Which restriction enzymes do cut single-stranded DNA ? (HindIII works).