Path: utzoo!attcan!uunet!mcsun!sunic!tut!hydra!hylka!aschulman
From: aschulman@cc.helsinki.fi
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: visualizing small pcr fragments
Message-ID: <1898.25d6f881@cc.helsinki.fi>
Date: 12 Feb 90 17:55:13 GMT
References: <136@uncmed.med.unc.edu>
Followup-To: bionet.molbio.methds-reagnts
Organization: University of Helsinki
Lines: 31

In article <136@uncmed.med.unc.edu>, danielg@uncmed.med.unc.edu writes:
> I have a small technical problem, perhaps one of you can give me a simple
> solution.
> 
> When I electrophorese my pcr products, I am looking for a band around 
> 200 bases long (pretty small).  The problem is that the band usually comes
> out right at the dye front, which makes UV visualization a slight problem.
> 
> Is there another dye I can use that will run a bit faster or slower than
> bromphenol blue?  I could try just using glycerol and edta in my loading
> buffer, leaving out the dye.
> 
> All suggestions welcome...
> 
> Daniel G. Sinclair
> Rsch Tech II
> University of NC, Chapel Hill
> 
>  Disclaimer: My opinion means | 'If you only knew how much I was holding
>              nothing, but His |  back, you would commend me'
>              means everything.|      - Charles Spurgeon, 19th century
>                               |        evangelist (on humor in preaching)





You can use xylene cyanol (Sigma X0377 and many other suppliers) which runs
more slowly than bromophenol at about .05% final concentration.

ASCHULMAN@FINUH.BITNET or ASCHULMAN@CC.HELSINKI.FI