Path: utzoo!attcan!uunet!mailrus!iuvax!purdue!haven!uvaarpa!mcnc!ecsvax.uncecs.edu!uncmed!earl!danielg
From: danielg@earl.med.unc.edu (Daniel Gene Sinclair)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Two Questions ... phenol/chloroform alternatives
Message-ID: <146@uncmed.med.unc.edu>
Date: 13 Feb 90 19:23:05 GMT
References: <900210080010179.WIBK@CSUGREEN.UCC.COLOSTATE.EDU>
Sender: news@uncmed.med.unc.edu
Reply-To: danielg@uncmed.med.unc.edu (Daniel Gene Sinclair)
Organization: UNC-CH School of Medicine
Lines: 20

We have just swithed to a non organic dna isolation procedure which is 
becoming common... common enough to be out in kit form (Oncor).

The protein is removed in a precipitation step (saturated NaCl) instead
of phenol/chloroform extraction.  We ran it along side of a p/c extraction
from whole blood and lymph nodes (chopped), and found yeilds and purity
identical, and found that we had much less problems with restriction of 
dna with the non-organic dna's.

No more phenol, hurray!  Let me know if you need more info, or call Oncor
at 301-963-3500.

-- dan

__________________________________________________________________________

 Disclaimer: My opinion means | 'If you only knew how much I was holding
             nothing, but His |  back, you would commend me'
             means everything.|      - Charles Spurgeon, 19th century
                              |        evangelist (on humor in preaching)